Liquid Based Separations Combined With Mass Spectrometric Detection

Speakers: Jonas Bergquist, My Moberg, Per Sjöberg and Aida Zuberovic

Today, mass spectrometry is established as one of the most important detection systems in chemical analysis and new possibilities are continuously added to the technique. It is now possible to analyze labile compounds, sub-femtomole concentrations, large and small biopolymeric substances, single cell content and non-covalent complexes. Besides the refinement of ion transport through different mass analyzers (i.e. quadrupoles, ion traps, time-of-flight (TOF) and ion cyclotron resonance analyzers (FT-ICR)) the main tools for the breakthrough of mass spectrometry in pharmaceutical and biomedical sciences are the ionization methods electrospray (ESI), atmospheric pressure chemical ionization (APCI) and matrix-assisted laser desorption (MALDI). Knowledge on the combined requirements for separation, ionization and mass analysis for samples in different matrices are today a prerequisite for a successful analysis.

This course in integrated liquid separation and mass spectrometry will give you an understanding of the tools available today in separation and mass spectrometric detection.

Course Topics:

• Ionization principles for Mass Spectrometry
• Capillary electrophoresis – Mass Spectrometry
• Liquid chromatography – Mass Spectrometry
• Lab-on-a-chip - Mass Spectrometry
• The analysis of complex samples

Target Audience :
The course is dedicated to technicians and chemists responsible for method development, analytical chemists, laboratory supervisors and scientists in the pharmaceutical and clinical setting but also in other fields of life sciences.