Preparative chromatography
Speaker: Attila Felinger, Robert Arnell
A course for those who want to learn more about preparative separations
- Introduction (60 min)
- Break (15 min)
- Methods for isotherm determination (60 min)
- Break (15 min)
- Optimisation of the preparative process (60 min)
The aim of the course is to provide the chromatographer with a deeper understanding of the separation process with emphasis on the optimisation of the preparative processes. The first hour will cover the fundamentals concerning the special operational conditions of preparative chromatography such as nonlinear chromatography; column overloading and general effects on the binary band profile due to competition will be discussed. In the second hour, the different methods for the acquisition of isotherm data – the essential input data for the optimisation – will be outlined and discussed both theoretically and practically. The methods are: frontal analysis (FA), the perturbation method (PM), and the inverse method (IM). In the last hour, the efficient optimisation of preparative chromatography – taking into account the modelling and engineering issues –will be discussed.
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