Enantioseparations

Speakers: by M. Lämmerhofer ^a, D. Armstrong ^b
a Institute of Analytical Chemistry, University of Vienna, Austria
^b Department of Chemistry, Iowa State University, Ames, IA, USA

The field of separation and analysis of enantiomers has become of prime importance in drug discovery and analytical quality control of chiral compounds, but has also growing relevance in preparative production of single enantiomers. A wide variety of technologies are available for these purposes, however, it is still hard to foresee which separation technology and chiral selector system provides the best chance to solve a given problem. In any case, a profound understanding of the underlying chiral recognition principles as well as separation mechanisms is required. In addition, practical aspects how to optimally integrate the separation into the total analysis concept including sample preparation and method validation are to be considered too.

This workshop will focus on liquid chromatographic enantiomer separation. After a general discussion of basic concepts and principles, an overview of the most frequently used chiral selectors and chiral stationary phases will be given. Particular focus will be laid on the discussion of recent new developments in the field. Some strategies for column selection will be presented. Another focus will be the application of liquid chromatographic enantiomer separations e.g. in bioanalytical assays. Guidance how to set-up and what to consider in the development of such methods will be provided including method validation aspects. The integral part are discussions of real case scenarios and potentially arising problems.